pbluescript skii vector (Agilent technologies)
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Agilent technologies
pbluescript skii vector
Pbluescript Skii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+skii%E2%88%92+vector/pm36250895-277-2-9
Average 90 stars, based on 1 article reviews
Pbluescript Skii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+skii%E2%88%92+vector/pm36250895-277-2-9
Average 90 stars, based on 1 article reviews
pbluescript skii vector - by Bioz Stars,
2026-10
90/100 stars
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Polymerase Chain Reaction:Article Title: Cloning and Characterization of Two NAD Kinases from Arabidopsis. Identification of a Calmodulin Binding Isoform Article Snippet: Template cDNA for PCR reactions was prepared by reverse transcribing total mRNA isolated from 14-d-old total seedling tissue as described below under the heading “RT-PCR.” Amplified NADK2 cDNA was ligated into a Sma I-digested pBluescript SKII− vector (Stratagene, La Jolla, CA) and then subsequently excised and subcloned into an Nde I and Sal I-digested pET 29a expression vector (Novagen, Madison, WI). Isolation:Article Title: Cloning and Characterization of Two NAD Kinases from Arabidopsis. Identification of a Calmodulin Binding Isoform Article Snippet: Template cDNA for PCR reactions was prepared by reverse transcribing total mRNA isolated from 14-d-old total seedling tissue as described below under the heading “RT-PCR.” Amplified NADK2 cDNA was ligated into a Sma I-digested pBluescript SKII− vector (Stratagene, La Jolla, CA) and then subsequently excised and subcloned into an Nde I and Sal I-digested pET 29a expression vector (Novagen, Madison, WI). Amplification:Article Title: Cloning and Characterization of Two NAD Kinases from Arabidopsis. Identification of a Calmodulin Binding Isoform Article Snippet: Template cDNA for PCR reactions was prepared by reverse transcribing total mRNA isolated from 14-d-old total seedling tissue as described below under the heading “RT-PCR.” Amplified NADK2 cDNA was ligated into a Sma I-digested pBluescript SKII− vector (Stratagene, La Jolla, CA) and then subsequently excised and subcloned into an Nde I and Sal I-digested pET 29a expression vector (Novagen, Madison, WI). Plasmid Preparation:Article Title: Cloning and Characterization of Two NAD Kinases from Arabidopsis. Identification of a Calmodulin Binding Isoform Article Snippet: Template cDNA for PCR reactions was prepared by reverse transcribing total mRNA isolated from 14-d-old total seedling tissue as described below under the heading “RT-PCR.” Amplified NADK2 cDNA was ligated into a Sma I-digested pBluescript SKII− vector (Stratagene, La Jolla, CA) and then subsequently excised and subcloned into an Nde I and Sal I-digested pET 29a expression vector (Novagen, Madison, WI). Expressing:Article Title: Cloning and Characterization of Two NAD Kinases from Arabidopsis. Identification of a Calmodulin Binding Isoform Article Snippet: Template cDNA for PCR reactions was prepared by reverse transcribing total mRNA isolated from 14-d-old total seedling tissue as described below under the heading “RT-PCR.” Amplified NADK2 cDNA was ligated into a Sma I-digested pBluescript SKII− vector (Stratagene, La Jolla, CA) and then subsequently excised and subcloned into an Nde I and Sal I-digested pET 29a expression vector (Novagen, Madison, WI). |